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| Registros recuperados: 12 | |
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| Castagna,Sandra Maria Ferraz; Muller,Monika; Macagnan,Marisa; Rodenbusch,Carla Rosane; Canal,Cláudio Wageck; Cardoso,Marisa. |
| The aim of this study was to compare a polymerase chain reaction (PCR) method combined with selective enrichment in Rappaport-Vassiliadis broth (PCR-RVB) with standard microbiological techniques (SMT) for the generic detection of Salmonella in samples of porcine origin. Two hundred sixty eight field samples consisting of 42 sets of pooled porcine mandibular lymph nodes and tonsils, 44 samples of intestinal content, 38 pork sausage meat samples and 144 samples of feed collected from swine farms were submitted to the PCR-RVB and SMT protocols. Salmonella was detected in 54 samples using the PCR-RVB assay and in 42 samples by SMT, three of the SMT Salmonella-positive samples (one each of S. Derby, S. Panama and S. Typhimurium) being Salmonella-negative by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Detection; PCR; Pork; Salmonella; Swine. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400013 |
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| Oliveira,Sílvia Dias de; Rodenbusch,Carla Rosane; Michael,Geovana B.; Cardoso,Marisa I.R.; Canal,Cláudio Wageck; Brandelli,Adriano. |
| The presence of three virulence genes, invA, spvR, and spvC, was determined in Salmonella Enteritidis isolated from poultry, pigs, humans and food. All isolates were positive for the invA gene, with 91.2% being positive for spvR and 90.2% for spvC. There was no significant difference in the prevalence of the virulence genes between isolates from different sources. The results indicate that there is a putative high virulence potential for the S. Enteritidis isolates characterized. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella Enteritidis; Virulence; Spv; PCR. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500042 |
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| Kindlein,Giselle; Ribeiro,Andréa Machado Leal; Canal,Cláudio Wageck; Vieira,Maitê de Moraes. |
| The effects of vitamin E and selenium (Se) supplementation on the immunity of hens vaccinated against a mixture of six swine-pathogenic Escherichia coli (EC) and avian encephalomyelitis virus (AEV) were studied. Antibody production (AbP) was evaluated in ninety 49 to 57-week-old H&N Nick Chick hens fed diets containing 14IU Vitamin E kg-1 (basal diet), 27, 59, 111, or 111IU vitamin E kg-1 + 0.56ppm Se supplementation. At 51 wks of age, half of the hens were vaccinated against EC, and all birds were vaccinated against AEV. At 53-weeks of age, the birds received a second dose of EC vaccine. Blood samples were collected weekly and serum was analyzed by ELISA for anti-EC IgY and was expressed as optical density (OD). Vaccinated hens had higher serum OD... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Avian encephalomyelitis; Escherichia coli; Immunoglobulin; Selenium; Vitamin E. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000500024 |
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| Streck,André Felipe; Souza,Carine Kunzler de; Gonçalves,Karla Rathje; Zang,Luciana; Pinto,Luciane Dubina; Canal,Cláudio Wageck. |
| The presence of canine parvovirus type 2 (CPV-2), 2a and 2b has been described in Brazil, however, the type 2c had not been reported until now. In the current study, seven out of nine samples from dogs with diarrhea were characterized as CPV-2c, indicating that this virus is already circulating in the Brazilian canine population. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Canine parvovirus type 2c; Sequence analysis; Brazil. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300008 |
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| Oliveira,Sílvia Dias de; Bessa,Marjo Cadó; Santos,Luciana Ruschel dos; Cardoso,Marisa Ribeiro de Itapema; Brandelli,Adriano; Canal,Cláudio Wageck. |
| In order to study the epidemiology of Salmonella Enteritidis outbreaks and determine the source of contamination so that a recurrence can be avoided, detailed characterization is necessary. Thus, the purpose of this study was to verify whether rep-PCR was able to discriminate among Salmonella Enteritidis isolates. Phage typing, detection of virulence genes and antimicrobial resistance testing were also associated to rep-PCR results. One hundred and two S. Enteritidis isolates from broiler carcasses, food, human, pigs, poultry-related samples, and nine isolates from other countries were genotypically typed by REP-PCR, ERIC-PCR and BOX-PCR, collectively called rep-PCR. Phage typing, detection of virulence genes and antimicrobial resistance testing were also... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella Enteritidis; Rep-PCR; Phage typing; Antimicrobial resistance; Virulence genes. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400025 |
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| Souza,Carine Kunzler; Streck,André Felipe; Gonçalves,Karla Ratje; Pinto,Luciane Dubina; Ravazzolo,Ana Paula; Barcellos,David Emílio dos Santos Neves de; Canal,Cláudio Wageck. |
| Abstract Ungulate tetraparvovirus 2 (UTV2) , formerly known as porcine hokovirus due to its discovery in Hong Kong, is closely related to a Primate tetraparvovirus (human PARV-4) and Ungulate tetraparvovirus 1 (bovine hokovirus). Until now, UTV2 was detected in European, Asian and North American countries, but its occurrence in Latin America is still unknown. This study describes the first report of UTV2 in Brazil, as well as its phylogenetic characterization. Tissue samples (lymph node, lung, liver, spleen and kidney) of 240 piglets from eight different herds (30 animals each herd) were processed for DNA extraction. UTV2 DNA was detected by PCR and the entire VP1/VP2 gene was sequenced for phylogenetic analysis. All pigs from this study displayed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Porcine; Tetraparvovirus; Phylogeny; Hokovirus; Detection; Ungulate. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200513 |
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| Ribeiro,Andréa Machado Leal; Rudnik,Liliane; Canal,Cláudio Wageck; Kratz,Lilian Ribeiro; Farias,Carolina. |
| Foi avaliado o efeito das gemas de ovos de aves hiperimunizadas contra Escherichia coli (E. coli) patogênica para suínos sobre a imunidade passiva de leitões recém-nascidos em uma unidade produtora de leitões. Foram avaliados a densidade ótica do ELISA (DO) para anticorpos contra E. coli, o peso corporal e a ocorrência de diarréia diária em 137 leitões recém-nascidos oriundos de 25 fêmeas primíparas, não vacinadas contra E. coli. As porcas foram consideradas blocos. De cada fêmea, foram separados seis leitões recém-nascidos de ambos os sexos, excluindo-se os mais leves e os mais pesados, divididos em três tratamentos e duas repetições. Os seguintes tratamentos foram fornecidos via oral: T1: 2 mL de PBS (tratamento controle) em duas doses, a primeira ao... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diarréia; Escherichia coli; Gema; Imunoglobulina Y; Imunidade passiva; Leitões. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982005000400019 |
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| Registros recuperados: 12 | |
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